5x first strand buffer (Thermo Fisher)
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5x first strand buffer
5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+first+strand+buffer/TRIS-HCL/pm41628766-56-27-51
Average 99 stars, based on 1 article reviews
5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+first+strand+buffer/TRIS-HCL/pm41628766-56-27-51
Average 99 stars, based on 1 article reviews
5x first strand buffer - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: Isolation, characterization, and host specificity of Solenopsis invicta virus 6 (Cripavirus porteri), a dicistrovirus from the red imported fire ant, Solenopsis invicta. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. 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Each well then received 5.4 μL of reverse transcription mix containing Superscript II reverse transcriptase (50 units) (Thermo Fisher), RNAse inhibitor (10 units) (Tarka Bio), Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), Article Title: Patient-Derived Medullary Thyroid Cancer Organoids: A Potential Model for Mechanistic Studies on Diagnostics and Therapy Article Snippet: Total RNA from patient material (n=3) and organoids (n=3) was extracted (RNeasyTM Mini Kit, Qiagen). .. To obtain cDNA, 500ng of total RNA was reverse transcribed using 1 μL 10 mM dNTP Mix, 100 ng random primers, Article Title: The AMPK-related kinase NUAK1 regulates neuronal morphogenesis through the RNA splicing co-factor SON Article Snippet: RNA concentration and purity were determined using the NanoDrop 2000 Spectrophotometer (ThermoScientific). .. Reverse transcription was performed mixing 500 ng of RNA with 10 mM dNTPs (EuroMedex, EUA01601, final concentration 500 μM), OligodT 12-18 primer (Invitrogen, 18418012, final mass 25 μg), 10 μM DTT and Random Hexamer:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), Virus:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), Gene Expression:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), Quantitative RT-PCR:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), SYBR Green Assay:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. After heat inactivation of DNase I with EDTA for 10 min at 65 °C, 0.5–1 μg RNA was incubated with a 4:1 mixture of oligo(dT) primers and random hexamer at 68 °C for 10 min. RNA/primers were incubated with Moloney murine leukemia virus reverse transcription (M-MLV RT) (Thermo Fisher Scientific), Article Title: Patient-Derived Medullary Thyroid Cancer Organoids: A Potential Model for Mechanistic Studies on Diagnostics and Therapy Article Snippet: Total RNA from patient material (n=3) and organoids (n=3) was extracted (RNeasyTM Mini Kit, Qiagen). .. To obtain cDNA, 500ng of total RNA was reverse transcribed using 1 μL 10 mM dNTP Mix, 100 ng random primers, Real-time Polymerase Chain Reaction:Article Title: Oncogenic DEAD-box ATPase DDX41 establishes transcript ensembles via CLK3-dependent and -independent mechanisms Article Snippet: Total RNA was purified from 2 to 5 ×10 cells with TRIzol (Invitrogen) and 1–2 μg RNA was treated with DNase I (Thermo Fisher Scientific) for 15 min at room temperature. .. 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Extension buffer containing 4 μl Concentration Assay:Article Title: The AMPK-related kinase NUAK1 regulates neuronal morphogenesis through the RNA splicing co-factor SON Article Snippet: RNA concentration and purity were determined using the NanoDrop 2000 Spectrophotometer (ThermoScientific). .. Reverse transcription was performed mixing 500 ng of RNA with 10 mM dNTPs (EuroMedex, EUA01601, final concentration 500 μM), OligodT 12-18 primer (Invitrogen, 18418012, final mass 25 μg), 10 μM DTT and |